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Keygen Biotech bca protein quantification kit
Bca Protein Quantification Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bca+kit/assay+bca+kit+protein/pmc13264244-191-6-13
Average 86 stars, based on 1 article reviews
bca protein quantification kit - by Bioz Stars, 2026-11
86/100 stars

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ALP Assay:

Article Title: Multifunctional bilayer scaffolds integrating melt electrowriting fibers and drug-loaded microspheres promote posteochondral regeneration through PI3K–AKT–mediated chondro-osteogenic signaling
Article Snippet: .. Chromogenic ALP readout was performed on day 14 by developing with BCIP/NBT for 30 min. For quantitative evaluation, cells were lysed in 1 % Triton X-100 for 30 min; lysates were clarified (12,000 rpm, 10 min, 4 °C) and the supernatants analyzed for total protein with the BCA kit (Keygen, China). .. On day 21, matrix mineralization was assessed using the ARS kit: Fixation was carried out in 4 % paraformaldehyde for 30 min at room temperature before staining, after which cells were washed in PBS; mineralized nodules were imaged microscopically.

BIA-KA:

Article Title: Multifunctional bilayer scaffolds integrating melt electrowriting fibers and drug-loaded microspheres promote posteochondral regeneration through PI3K–AKT–mediated chondro-osteogenic signaling
Article Snippet: .. Chromogenic ALP readout was performed on day 14 by developing with BCIP/NBT for 30 min. For quantitative evaluation, cells were lysed in 1 % Triton X-100 for 30 min; lysates were clarified (12,000 rpm, 10 min, 4 °C) and the supernatants analyzed for total protein with the BCA kit (Keygen, China). .. On day 21, matrix mineralization was assessed using the ARS kit: Fixation was carried out in 4 % paraformaldehyde for 30 min at room temperature before staining, after which cells were washed in PBS; mineralized nodules were imaged microscopically.

Protein Extraction:

Article Title: CRISPLD2 protects against liver inflammation and fibrosis via GRP78 to repress HMGB1/TLR4 axis–mediated STING palmitoylation
Article Snippet: The levels of IL-6, IL-1β, and TNF-α in the supernatant of primary hepatocytes and mouse serum were detected using the Mouse IL-6 ELISA Kit (SEKM-0007), Mouse IL-1β ELISA Kit (SEKM-0002; Solarbio, Beijing, China), and Mouse TNF-α ELISA Kit (SEKM-0034) from Solarbio (Beijing, China). .. Total protein was extracted from primary hepatocytes and JS-1 cells using the protein extraction kit (KGB5303; KeyGEN, Nanjing, China) and quantified using a BCA kit (KGB2101; KeyGEN). .. The protein samples were separated with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), blotted to polyvinylidene fluoride membranes, blocked in 5% skim milk for 1 hour, and incubated with primary antibodies against α-SMA (ab7817, 1:1000; Abcam), FN (ab2413, 1:1000; Abcam), col1a1 (ab279711, 1:1000; Abcam), CRISPLD2 (orb478011, 1:500; Biorbyt, NC, USA), TLR4 (MA516216, 1:1000; Thermo Fisher Scientific, MA, USA), ATG3 (720056, 1:1000; Thermo Fisher Scientific), ATG7 (ab133528, 1:10000; Abcam), ATG12 (ab155589, 1:1000; Abcam), ATG16L1 (ab233796, 1:500; Abcam), LC3 (ab192890, 1:2000; Abcam), p62 (ab109012, 1:10000; Abcam), STING (PA5-83404, 1:1000; Thermo Fisher Scientific), GRP78 (MA5-27686, 1:2000, Thermo Fisher Scientific), PCNA (ab29, 1:1000, Abcam), Calnexin (ab22595, 1:1000; Abcam), and β-actin (ab6276, 1:5000; Abcam) overnight at 4°C.

Incubation:

Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity
Article Snippet: The mPFC tissue were lysed with RIPA lysis buffer (Beyotime, No. P0013B) and were homogenized with a freeze grinder (Jingxin, Shanghai, China) for 3 min, 90 hz. .. After incubation in 4 °C for 1 h, the samples were centrifuged at 12,000 × g and collect the supernatant to determine protein concentration with a BCA kit (KeyGen Biotech, China, No. KGP903). ..

Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity.
Article Snippet: The mPFC tissue were lysed with RIPA lysis buffer (Beyotime, No. P0013B) and were homogenized with a freeze grinder (Jingxin, Shanghai, China) for 3 min, 90 hz. .. After incubation in 4 °C for 1 h, the samples were centrifuged at 12,000 × g and collect the supernatant to determine protein concentration with a BCA kit (KeyGen Biotech, China, No. KGP903). ..

Protein Concentration:

Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity
Article Snippet: The mPFC tissue were lysed with RIPA lysis buffer (Beyotime, No. P0013B) and were homogenized with a freeze grinder (Jingxin, Shanghai, China) for 3 min, 90 hz. .. After incubation in 4 °C for 1 h, the samples were centrifuged at 12,000 × g and collect the supernatant to determine protein concentration with a BCA kit (KeyGen Biotech, China, No. KGP903). ..

Article Title: Knockdown of Ddx3x in mPFC induces autistic-like phenotype in mice via altered synaptic plasticity.
Article Snippet: The mPFC tissue were lysed with RIPA lysis buffer (Beyotime, No. P0013B) and were homogenized with a freeze grinder (Jingxin, Shanghai, China) for 3 min, 90 hz. .. After incubation in 4 °C for 1 h, the samples were centrifuged at 12,000 × g and collect the supernatant to determine protein concentration with a BCA kit (KeyGen Biotech, China, No. KGP903). ..



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